pFastBac-GFP-dynein-2(D1091–Q4307)
(Plasmid #64064)

• Purpose: Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
• Depositing Lab: Andrew Carter
• Publication: Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi: 10.1038/nature14023. Epub 2014 Dec 1.

Backbone

• Vector backbone: pFastBac-1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4707
• Total vector size (bp): 15606
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cytoplasmic dynein-2
• Alt name: Cytoplasmic dynein 2
• Alt name: IFT dynein
• Species: H. sapiens (human)
• Insert Size (bp): 10899
• Mutation: Deleted amino acids 1-1090, extra C-terminal valine (V4307) added
• Entrez Gene: DYNC2H1 (a.k.a. ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11)
• Promoter: Polyhedrin
• Tags / Fusion Proteins:
  • His tag (N terminal on insert)
  • ZZ tag (N terminal on insert)
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GCCTTCATCCGTTTCCAC
• 3′ sequencing primer: CCTGCTCAACCAGAAGTGC

Resource Information

• Supplemental Documents:
  • pFastBac-GFP-dynein-2_D1091–Q4307
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's quality control sequencing identified one E to K amino acid residue substitution in the ZZ tag. The depositing laboratory does not expect this residue change to affect tag function.