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pDyn1
(Plasmid #64067)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64067 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    EMBL
  • Backbone size w/o insert (bp) 3938
  • Total vector size (bp) 17876
  • Vector type
    Insect Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DYNC1H1
  • Alt name
    Cytoplasmic dynein heavy chain 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    13938
  • Mutation
    Codon optimized for Sf9
  • GenBank ID
    NM_001376.4
  • Entrez Gene
    DYNC1H1 (a.k.a. CDCBM13, CMT2O, DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, SMALED1, p22)
  • Tag / Fusion Protein
    • His-ZZ-TCS-SNAPf (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDyn1 was a gift from Andrew Carter (Addgene plasmid # 64067 ; http://n2t.net/addgene:64067 ; RRID:Addgene_64067)
  • For your References section:

    In vitro reconstitution of a highly processive recombinant human dynein complex. Schlager MA, Hoang HT, Urnavicius L, Bullock SL, Carter AP. EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. Epub 2014 Jul 1. 10.15252/embj.201488792 PubMed 24986880