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CrBlue-mcherry - pcDNA3.1
(Plasmid #64204)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64204 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7082
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CrBlue
  • Species
    Synthetic
  • Insert Size (bp)
    971
  • Promoter CMV
  • Tag / Fusion Protein
    • mcherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CrBlue was cloned into the EcoR1 and NotI sites of pcDNA3.1 and mcherry was fused to the C terminus of CrBlue using the NotI and XbaI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CrBlue-mcherry - pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 64204 ; http://n2t.net/addgene:64204 ; RRID:Addgene_64204)
  • For your References section:

    Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Karunarathne WK, Giri L, Kalyanaraman V, Gautam N. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 10.1073/pnas.1220697110. Epub 2013 Mar 11. 10.1073/pnas.1220697110 PubMed 23479634