PurposeGFP-tagged mammalian expression of Nup153
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64268||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 9127
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameNucleoporin 153kDa
SpeciesH. sapiens (human)
Entrez GeneNUP153 (a.k.a. HNUP153, N153)
- Promoter CMV
/ Fusion Protein
- GFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XmaI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP(C3)-Nup153 was a gift from Birthe Fahrenkrog (Addgene plasmid # 64268 ; http://n2t.net/addgene:64268 ; RRID:Addgene_64268)
For your References section:Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy. Duheron V, Chatel G, Sauder U, Oliveri V, Fahrenkrog B. Nucleus. 2014;5(6):601-12. doi: 10.4161/19491034.2014.990853. 10.4161/19491034.2014.990853 PubMed 25485891
Map uploaded by the depositor.