pAc-­His-­zfTpo
(Plasmid #64309)

• Purpose: Express soluble His-tagged zebrafish thrombopoietin in insect cells
• Depositing Lab: Petr Bartunek
• Publication: Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10.1182/blood-2014-03-564682. Epub 2014 May 28.

Backbone

• Vector backbone: pAcGP67-­B
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 9765
• Total vector size (bp): 10255
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Thrombopoietin
• Alt name: tpo
• Alt name: thpo
• Species: D. rerio (zebrafish)
• Mutation: Thrombopoietin starts at AA37 (AA1-36 encode the signal peptide which is not present in the mature secreted protein)
• GenBank ID: EU267076.1
• Entrez Gene: thpo (a.k.a. T, tpo)
• Tag / Fusion Protein:
  • (His)6 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: AAA TGA TAA CCA TCT CGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry