pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)
(Plasmid #64314)

• Purpose: chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells
• Depositing Lab: Noriyuki Tsumaki
• Publication: Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi: 10.1371/journal.pone.0077365. eCollection 2013.

Backbone

• Vector backbone: pLe6Δ (P4-32)
• Modifications to backbone: deletion of the PGKpromoter-EM7-Blastcidine sequence at KpnI sites from pLenti6.4/R4R2/V5-DEST (Invitrogen).
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: COL11A2 promoter
• Alt name: COL11A2, collagen, Type XI, alpha 2
• Species: H. sapiens (human)
• Insert Size (bp): 6013
• Mutation: N/A
• Entrez Gene: COL11A2 (a.k.a. DFNA13, DFNB53, FBCG2, HKE5, OSMEDA, OSMEDB, PARP, STL3)
• Tags / Fusion Proteins:
  • EGFP (C terminal on insert)
  • IRES-PURO (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: EGFP-N

Gene/Insert 2

• Gene/Insert name: COL11A2 enhancer
• Alt name: COL11A2, collagen, Type XI, alpha 2
• Species: H. sapiens (human)
• Mutation: N/A
• Entrez Gene: COL11A2 (a.k.a. DFNA13, DFNB53, FBCG2, HKE5, OSMEDA, OSMEDB, PARP, STL3)

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Puro-F2 ctcggcttcaccgtcacc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gateway cassette was purchased from Invitrogen.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry