pTLR repair vector
(Plasmid #64322)

• Purpose: Vector for repair of the traffic light reporter (TLR), providing the venus coding region
• Depositing Lab: Ralf Kuehn
• Publication: Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.

Backbone

• Vector backbone: Bluescript
• Vector type: Artificial targeting vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Venus
• Alt name: Venus repair vector
• Alt name: pVenus donor
• Species: Aequorea victoria
• Mutation: see publication for details
• GenBank ID: L29345.1
• Promoter: none

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13pUC-rev
• 3′ sequencing primer: M13pUC-fwd

Resource Information

• Supplemental Documents:
  • Plasmid gb file
  • Plasmid sequence in genbank format
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry