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pTLR repair vector
(Plasmid #64322)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64322 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Venus
  • Alt name
    Venus repair vector
  • Alt name
    pVenus donor
  • Species
    Aequorea victoria
  • Mutation
    see publication for details
  • GenBank ID
    L29345.1
  • Promoter none

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTLR repair vector was a gift from Ralf Kuehn (Addgene plasmid # 64322 ; http://n2t.net/addgene:64322 ; RRID:Addgene_64322)
  • For your References section:

    Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. 10.1038/nbt.3198 PubMed 25803306