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pU6-(BbsI)_CBh-Cas9-T2A-mCherry Citations (37)

Plasmid Article: Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
PubMed Journal

Articles Citing pU6-(BbsI)_CBh-Cas9-T2A-mCherry

Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier. Vatine GD, Al-Ahmad A, Barriga BK, Svendsen S, Salim A, Garcia L, Garcia VJ, Ho R, Yucer N, Qian T, Lim RG, Wu J, Thompson LM, Spivia WR, Chen Z, Van Eyk J, Palecek SP, Refetoff S, Shusta EV, Svendsen CN. Cell Stem Cell. 2017 Jun 1;20(6):831-843.e5. doi: 10.1016/j.stem.2017.04.002. Epub 2017 May 16. PubMed
Nuclear Envelope Rupture Is Enhanced by Loss of p53 or Rb. Yang Z, Maciejowski J, de Lange T. Mol Cancer Res. 2017 Nov;15(11):1579-1586. doi: 10.1158/1541-7786.MCR-17-0084. Epub 2017 Aug 15. PubMed
Sodium Channel beta2 Subunits Prevent Action Potential Propagation Failures at Axonal Branch Points. Cho IH, Panzera LC, Chin M, Hoppa MB. J Neurosci. 2017 Sep 27;37(39):9519-9533. doi: 10.1523/JNEUROSCI.0891-17.2017. Epub 2017 Sep 4. PubMed
Widespread activation of antisense transcription of the host genome during herpes simplex virus 1 infection. Wyler E, Menegatti J, Franke V, Kocks C, Boltengagen A, Hennig T, Theil K, Rutkowski A, Ferrai C, Baer L, Kermas L, Friedel C, Rajewsky N, Akalin A, Dolken L, Grasser F, Landthaler M. Genome Biol. 2017 Oct 31;18(1):209. doi: 10.1186/s13059-017-1329-5. PubMed
Monitoring of Dual CRISPR/Cas9-Mediated Steroidogenic Acute Regulatory Protein Gene Deletion and Cholesterol Accumulation Using High-Resolution Fluorescence In Situ Hybridization in a Single Cell. Lee J, Jefcoate C. Front Endocrinol (Lausanne). 2017 Oct 25;8:289. doi: 10.3389/fendo.2017.00289. eCollection 2017. PubMed
A Pan-Cancer Analysis of Enhancer Expression in Nearly 9000 Patient Samples. Chen H, Li C, Peng X, Zhou Z, Weinstein JN, Liang H. Cell. 2018 Apr 5;173(2):386-399.e12. doi: 10.1016/j.cell.2018.03.027. PubMed
Fast-Evolving Human-Specific Neural Enhancers Are Associated with Aging-Related Diseases. Chen H, Li C, Zhou Z, Liang H. Cell Syst. 2018 May 23;6(5):604-611.e4. doi: 10.1016/j.cels.2018.04.002. PubMed
IMP/GTP balance modulates cytoophidium assembly and IMPDH activity. Keppeke GD, Chang CC, Peng M, Chen LY, Lin WC, Pai LM, Andrade LEC, Sung LY, Liu JL. Cell Div. 2018 Jun 15;13:5. doi: 10.1186/s13008-018-0038-0. eCollection 2018. PubMed
Viral diversity is an obligate consideration in CRISPR/Cas9 designs for targeting the HIV reservoir. Roychoudhury P, De Silva Feelixge H, Reeves D, Mayer BT, Stone D, Schiffer JT, Jerome KR. BMC Biol. 2018 Jul 11;16(1):75. doi: 10.1186/s12915-018-0544-1. PubMed
Long Non-Coding RNA Modulation of VEGF-A during Hypoxia. Nieminen T, Scott TA, Lin FM, Chen Z, Yla-Herttuala S, Morris KV. Noncoding RNA. 2018 Nov 20;4(4). pii: ncrna4040034. doi: 10.3390/ncrna4040034. PubMed
The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism. Mentink RA, Rella L, Radaszkiewicz TW, Gybel T, Betist MC, Bryja V, Korswagen HC. PLoS Genet. 2018 Dec 7;14(12):e1007840. doi: 10.1371/journal.pgen.1007840. eCollection 2018 Dec. PubMed
Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9. Jin YH, Joo H, Lee K, Kim H, Didier R, Yang Y, Shin H, Lee C. Sci Rep. 2019 Jan 22;9(1):277. doi: 10.1038/s41598-018-36736-y. PubMed
Loss of Neurofascin-186 Disrupts Alignment of AnkyrinG Relative to Its Binding Partners in the Axon Initial Segment. Alpizar SA, Baker AL, Gulledge AT, Hoppa MB. Front Cell Neurosci. 2019 Jan 22;13:1. doi: 10.3389/fncel.2019.00001. eCollection 2019. PubMed
Transposable elements drive widespread expression of oncogenes in human cancers. Jang HS, Shah NM, Du AY, Dailey ZZ, Pehrsson EC, Godoy PM, Zhang D, Li D, Xing X, Kim S, O'Donnell D, Gordon JI, Wang T. Nat Genet. 2019 Apr;51(4):611-617. doi: 10.1038/s41588-019-0373-3. Epub 2019 Mar 29. PubMed
Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1. Harnos J, Canizal MCA, Jurasek M, Kumar J, Holler C, Schambony A, Hanakova K, Bernatik O, Zdrahal Z, Gomoryova K, Gybel T, Radaszkiewicz TW, Kravec M, Trantirek L, Rynes J, Dave Z, Fernandez-Llamazares AI, Vacha R, Tripsianes K, Hoffmann C, Bryja V. Nat Commun. 2019 Apr 18;10(1):1804. doi: 10.1038/s41467-019-09651-7. PubMed
Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant. Nambiar TS, Billon P, Diedenhofen G, Hayward SB, Taglialatela A, Cai K, Huang JW, Leuzzi G, Cuella-Martin R, Palacios A, Gupta A, Egli D, Ciccia A. Nat Commun. 2019 Jul 30;10(1):3395. doi: 10.1038/s41467-019-11105-z. PubMed

Associated Plasmids

TRPM4 is highly expressed in human colorectal tumor buds and contributes to proliferation, cell cycle, and invasion of colorectal cancer cells. Kappel S, Stoklosa P, Hauert B, Ross-Kaschitza D, Borgstrom A, Baur R, Galvan JA, Zlobec I, Peinelt C. Mol Oncol. 2019 Nov;13(11):2393-2405. doi: 10.1002/1878-0261.12566. Epub 2019 Sep 12. PubMed
Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Reuven N, Adler J, Broennimann K, Myers N, Shaul Y. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. PubMed

Associated Plasmids

Co-opted transposons help perpetuate conserved higher-order chromosomal structures. Choudhary MN, Friedman RZ, Wang JT, Jang HS, Zhuo X, Wang T. Genome Biol. 2020 Jan 24;21(1):16. doi: 10.1186/s13059-019-1916-8. PubMed
A genetic toolbox for metabolic engineering of Issatchenkia orientalis. Cao M, Fatma Z, Song X, Hsieh PH, Tran VG, Lyon WL, Sayadi M, Shao Z, Yoshikuni Y, Zhao H. Metab Eng. 2020 May;59:87-97. doi: 10.1016/j.ymben.2020.01.005. Epub 2020 Jan 31. PubMed
Optimized CRISPR/Cas9 strategy for homology-directed multiple targeted integration of transgenes in CHO cells. Shin SW, Lee JS. Biotechnol Bioeng. 2020 Jun;117(6):1895-1903. doi: 10.1002/bit.27315. Epub 2020 Mar 2. PubMed
CRISPR/Cas9-Induced Loss of Keap1 Enhances Anti-oxidation in Rat Adipose-Derived Mesenchymal Stem Cells. Hu Y, Liu S, Zhu BM. Front Neurol. 2020 Feb 18;10:1311. doi: 10.3389/fneur.2019.01311. eCollection 2019. PubMed
Enhancement of homology-directed repair with chromatin donor templates in cells. Cruz-Becerra G, Kadonaga JT. Elife. 2020 Apr 28;9. pii: 55780. doi: 10.7554/eLife.55780. PubMed
Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells. Shin S, Kim SH, Shin SW, Grav LM, Pedersen LE, Lee JS, Lee GM. ACS Synth Biol. 2020 Jun 19;9(6):1263-1269. doi: 10.1021/acssynbio.0c00097. Epub 2020 Jun 2. PubMed
The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo. Bressan C, Pecora A, Gagnon D, Snapyan M, Labrecque S, De Koninck P, Parent M, Saghatelyan A. Elife. 2020 Sep 28;9. pii: 56006. doi: 10.7554/eLife.56006. PubMed
Pooled protein tagging, cellular imaging, and in situ sequencing for monitoring drug action in real time. Reicher A, Koren A, Kubicek S. Genome Res. 2020 Dec;30(12):1846-1855. doi: 10.1101/gr.261503.120. Epub 2020 Nov 17. PubMed
Cross-linking mass spectrometry reveals the structural topology of peripheral NuRD subunits relative to the core complex. Spruijt CG, Grawe C, Kleinendorst SC, Baltissen MPA, Vermeulen M. FEBS J. 2021 May;288(10):3231-3245. doi: 10.1111/febs.15650. Epub 2020 Dec 14. PubMed
Adult neural stem cell activation in mice is regulated by the day/night cycle and intracellular calcium dynamics. Gengatharan A, Malvaut S, Marymonchyk A, Ghareghani M, Snapyan M, Fischer-Sternjak J, Ninkovic J, Gotz M, Saghatelyan A. Cell. 2021 Feb 4;184(3):709-722.e13. doi: 10.1016/j.cell.2020.12.026. Epub 2021 Jan 21. PubMed
Hydroxyurea selection for enhancement of homology-directed targeted integration of transgenes in CHO cells. Kwak JM, Lee Y, Shin SW, Lee JS. N Biotechnol. 2021 May 25;62:26-31. doi: 10.1016/j.nbt.2021.01.007. Epub 2021 Jan 21. PubMed
Omnitemporal choreographies of all five STIM/Orai and IP3Rs underlie the complexity of mammalian Ca(2+) signaling. Emrich SM, Yoast RE, Xin P, Arige V, Wagner LE, Hempel N, Gill DL, Sneyd J, Yule DI, Trebak M. Cell Rep. 2021 Mar 2;34(9):108760. doi: 10.1016/j.celrep.2021.108760. PubMed
Primary Cilia Formation Does Not Rely on WNT/beta-Catenin Signaling. Bernatik O, Paclikova P, Kotrbova A, Bryja V, Cajanek L. Front Cell Dev Biol. 2021 Feb 26;9:623753. doi: 10.3389/fcell.2021.623753. eCollection 2021. PubMed
Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion. Gangoso E, Southgate B, Bradley L, Rus S, Galvez-Cancino F, McGivern N, Guc E, Kapourani CA, Byron A, Ferguson KM, Alfazema N, Morrison G, Grant V, Blin C, Sou I, Marques-Torrejon MA, Conde L, Parrinello S, Herrero J, Beck S, Brandner S, Brennan PM, Bertone P, Pollard JW, Quezada SA, Sproul D, Frame MC, Serrels A, Pollard SM. Cell. 2021 Apr 29;184(9):2454-2470.e26. doi: 10.1016/j.cell.2021.03.023. Epub 2021 Apr 14. PubMed
Candidate Alzheimer's Disease Biomarker miR-483-5p Lowers TAU Phosphorylation by Direct ERK1/2 Repression. Nagaraj S, Want A, Laskowska-Kaszub K, Fesiuk A, Vaz S, Logarinho E, Wojda U. Int J Mol Sci. 2021 Apr 1;22(7). pii: ijms22073653. doi: 10.3390/ijms22073653. PubMed
Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy. Koeniger A, Brichkina A, Nee I, Dempwolff L, Hupfer A, Galperin I, Finkernagel F, Nist A, Stiewe T, Adhikary T, Diederich W, Lauth M. Cancers (Basel). 2021 Apr 15;13(8). pii: cancers13081908. doi: 10.3390/cancers13081908. PubMed
Roles of individual human Dishevelled paralogs in the Wnt signalling pathways. Paclikova P, Radaszkiewicz TW, Potesil D, Harnos J, Zdrahal Z, Bryja V. Cell Signal. 2021 Sep;85:110058. doi: 10.1016/j.cellsig.2021.110058. Epub 2021 May 31. PubMed
Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes. Malvaut S, Marymonchyk A, Gengatharan A, Saghatelyan A. STAR Protoc. 2021 Jun 12;2(2):100596. doi: 10.1016/j.xpro.2021.100596. eCollection 2021 Jun 18. PubMed
Small tandem DNA duplications result from CST-guided Pol alpha-primase action at DNA break termini. Schimmel J, Munoz-Subirana N, Kool H, van Schendel R, Tijsterman M. Nat Commun. 2021 Aug 10;12(1):4843. doi: 10.1038/s41467-021-25154-w. PubMed

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