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Cas9-NAT Citations (15)

Originally described in: Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
PubMed Journal

Articles Citing Cas9-NAT

Articles
Metabolic Engineering of Probiotic Saccharomyces boulardii. Liu JJ, Kong II, Zhang GC, Jayakody LN, Kim H, Xia PF, Kwak S, Sung BH, Sohn JH, Walukiewicz HE, Rao CV, Jin YS. Appl Environ Microbiol. 2016 Apr 4;82(8):2280-7. doi: 10.1128/AEM.00057-16. Print 2016 Apr 15. PubMed
Enhanced isoprenoid production from xylose by engineered Saccharomyces cerevisiae. Kwak S, Kim SR, Xu H, Zhang GC, Lane S, Kim H, Jin YS. Biotechnol Bioeng. 2017 Jul 1. doi: 10.1002/bit.26369. PubMed
Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes. Barbieri EM, Muir P, Akhuetie-Oni BO, Yellman CM, Isaacs FJ. Cell. 2017 Nov 30;171(6):1453-1467.e13. doi: 10.1016/j.cell.2017.10.034. Epub 2017 Nov 16. PubMed
Improved squalene production through increasing lipid contents in Saccharomyces cerevisiae. Wei LJ, Kwak S, Liu JJ, Lane S, Hua Q, Kweon DH, Jin YS. Biotechnol Bioeng. 2018 Jul;115(7):1793-1800. doi: 10.1002/bit.26595. Epub 2018 Apr 6. PubMed
Enhanced acetic acid stress tolerance and ethanol production in Saccharomyces cerevisiae by modulating expression of the de novo purine biosynthesis genes. Zhang MM, Xiong L, Tang YJ, Mehmood MA, Zhao ZK, Bai FW, Zhao XQ. Biotechnol Biofuels. 2019 May 10;12:116. doi: 10.1186/s13068-019-1456-1. eCollection 2019. PubMed
Replication Fork Activation Is Enabled by a Single-Stranded DNA Gate in CMG Helicase. Wasserman MR, Schauer GD, O'Donnell ME, Liu S. Cell. 2019 Jul 25;178(3):600-611.e16. doi: 10.1016/j.cell.2019.06.032. PubMed
Vitamin A Production by Engineered Saccharomyces cerevisiae from Xylose via Two-Phase in Situ Extraction. Sun L, Kwak S, Jin YS. ACS Synth Biol. 2019 Sep 20;8(9):2131-2140. doi: 10.1021/acssynbio.9b00217. Epub 2019 Aug 20. PubMed
Identification and characterization of novel xylose isomerases from a Bos taurus fecal metagenome. Tang R, Ye P, Alper HS, Liu Z, Zhao X, Bai F. Appl Microbiol Biotechnol. 2019 Dec;103(23-24):9465-9477. doi: 10.1007/s00253-019-10161-1. Epub 2019 Nov 7. PubMed
High-level beta-carotene production from xylose by engineered Saccharomyces cerevisiae without overexpression of a truncated HMG1 (tHMG1). Sun L, Atkinson CA, Lee YG, Jin YS. Biotechnol Bioeng. 2020 Nov;117(11):3522-3532. doi: 10.1002/bit.27508. Epub 2020 Aug 6. PubMed
Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast. Sun L, Lee JW, Yook S, Lane S, Sun Z, Kim SR, Jin YS. Nat Commun. 2021 Aug 17;12(1):4975. doi: 10.1038/s41467-021-25241-y. PubMed
Chemical rescue of mutant proteins in living Saccharomyces cerevisiae cells by naturally occurring small molecules. Hassell DS, Steingesser MG, Denney AS, Johnson CR, McMurray MA. G3 (Bethesda). 2021 Sep 6;11(9). pii: 6323229. doi: 10.1093/g3journal/jkab252. PubMed
CRISPRi-Guided Metabolic Flux Engineering for Enhanced Protopanaxadiol Production in Saccharomyces cerevisiae. Lim SH, Baek JI, Jeon BM, Seo JW, Kim MS, Byun JY, Park SH, Kim SJ, Lee JY, Lee JH, Kim SC. Int J Mol Sci. 2021 Oct 31;22(21). pii: ijms222111836. doi: 10.3390/ijms222111836. PubMed
Selective production of retinol by engineered Saccharomyces cerevisiae through the expression of retinol dehydrogenase. Lee YG, Kim C, Sun L, Lee TH, Jin YS. Biotechnol Bioeng. 2022 Feb;119(2):399-410. doi: 10.1002/bit.28004. Epub 2021 Dec 11. PubMed
Dissection and enhancement of prebiotic properties of yeast cell wall oligosaccharides through metabolic engineering. Kwak S, Robinson SJ, Lee JW, Lim H, Wallace CL, Jin YS. Biomaterials. 2022 Mar;282:121379. doi: 10.1016/j.biomaterials.2022.121379. Epub 2022 Jan 18. PubMed
A widespread inversion polymorphism conserved among Saccharomyces species is caused by recurrent homogenization of a sporulation gene family. Salzberg LI, Martos AAR, Lombardi L, Jermiin LS, Blanco A, Byrne KP, Wolfe KH. PLoS Genet. 2022 Nov 28;18(11):e1010525. doi: 10.1371/journal.pgen.1010525. eCollection 2022 Nov. PubMed

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