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pUbiSXR
(Plasmid #64403)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64403 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP72
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    xylanase
  • Alt name
    sXynA
  • Species
    Synthetic; Neocallimastix patriciarum
  • Mutation
    codon optimized for plant expression
  • Promoter Plant ubiquitin (Ubi-1) promoter (with untranslated region intron)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plant ubiquitin (Ubi-1) promoter (with untranslated region intron) driving expression of a synthetic, codon-optimised xylanase gene followed by an rbcS terminator. The xylanase is used as a reporter gene for quantitative promoter expression in plant tissues.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUbiSXR was a gift from Claudia Vickers (Addgene plasmid # 64403 ; http://n2t.net/addgene:64403 ; RRID:Addgene_64403)
  • For your References section:

    A synthetic xylanase as a novel reporter in plants. Vickers CE, Xue GP, Gresshoff PM. Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4. 10.1007/s00299-003-0667-9 PubMed 12845475