PTEF1-yEGFPCLN2PEST-pRS406
(Plasmid #64406)

• Purpose: TEF promoter driven GFP expression
• Depositing Lab: Claudia Vickers
• Publication: Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7.

Backbone

• Vector backbone: GFPCLN2PEST-pRS406
• Backbone manufacturer: Mateus and Avery 2000
• Modifications to backbone: destabilized GFP
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TEF1 promoter
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: TEF1 (a.k.a. YPR080W)
• Promoter: TEF1 promoter
• Tag / Fusion Protein:
  • yEGFPCLN2PEST (destabilized yeast EGFP) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: yGFP-R

Resource Information

• Supplemental Documents:
  • PTEF1-yEGFPCLN2PEST-pRS406 genbank sequence
• A portion of this plasmid was derived from a plasmid made by: Euroscarf
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments