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pGL2-HKII-F
(Plasmid #64518)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64518 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Total vector size (bp) 5937
  • Modifications to backbone
    None
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SURE
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Type II hexokinase gene, partial cds and promoter region
  • Alt name
    HKII
  • Alt name
    HK2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    340
  • Mutation
    Isolated from AS-30D rat hepatoma
  • GenBank ID
    U19605.2
  • Entrez Gene
    Hk2
  • Promoter Hexokinase II promoter deleted
  • Tag / Fusion Protein
    • firefly luciferase

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GL primer 1
  • 3′ sequencing primer GL primer 2
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For stability the plasmid is maintained in E. coli SURE. For reporter studies it is propagated in E. coli SCS110 or other methylation deficient strain.

Note: Addgene's quality control sequencing has identified a few single nucleotide discrepancies with the depositor's reference sequence. However, the plasmid accurately reflects the construct as it was used in the associated publication, and should function as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-HKII-F was a gift from Peter Pedersen (Addgene plasmid # 64518 ; http://n2t.net/addgene:64518 ; RRID:Addgene_64518)
  • For your References section:

    Glucose catabolism in cancer cells: identification and characterization of a marked activation response of the type II hexokinase gene to hypoxic conditions. Mathupala SP, Rempel A, Pedersen PL. J Biol Chem. 2001 Nov 16;276(46):43407-12. Epub 2001 Sep 13. 10.1074/jbc.M108181200 PubMed 11557773