pLenti-NIrD
(Plasmid #65010)

• Purpose: NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection
• Depositing Lab: Wensheng Wei
• Publication: Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038/srep08865.

Backbone

• Vector backbone: pLenti-CMVMCSSVBsd
• Backbone size w/o insert (bp): 7578
• Modifications to backbone: The CMV promoter was replaced with the tight-TRE promoter and DNA fragments coding delta-TK (a modified version of HSV-TK), 2A (2A of the Thosea asigna virus) and mCherry were concatenated sequentially. rtTA-MAVS(C)-IRES was inserted between the SV40 promoter and the Blasticidin marker gene.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl4
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: rtTA-MAVS(C)
• Alt name: reverse tetracycline transactivator-mitochondrial antiviral signalling protein (C-term aa462-540), NS3-4A cleavage site
• Promoter: Tight-TRE

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: 2A-linked delta-TK
• Promoter: Tight-TRE
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry