Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMSCV-flag-myr-Akt1-IRES-GFP
(Plasmid #65063)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65063 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 8161
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Akt1
  • Alt name
    pkb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1661
  • Entrez Gene
    AKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Tags / Fusion Proteins
    • myristoylation tag (N terminal on insert)
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer MSCV LTR 5'
  • 3′ sequencing primer MSCV LTR 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

the plasmid is the property of Dana-Farber Cancer Institute

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-flag-myr-Akt1-IRES-GFP was a gift from Kira Gritsman & Jean Zhao (Addgene plasmid # 65063 ; http://n2t.net/addgene:65063 ; RRID:Addgene_65063)
  • For your References section:

    Constitutively active AKT depletes hematopoietic stem cells and induces leukemia in mice. Kharas MG, Okabe R, Ganis JJ, Gozo M, Khandan T, Paktinat M, Gilliland DG, Gritsman K. Blood. 2010 Feb 18;115(7):1406-15. doi: 10.1182/blood-2009-06-229443. Epub 2009 Dec 14. 10.1182/blood-2009-06-229443 PubMed 20008787