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pGL3-sbIL6P
(Plasmid #65099)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65099 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    interleukin-6 promoter
  • Alt name
    IL-6
  • Species
    Seabream (Sparus aurata)
  • Insert Size (bp)
    1100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer 3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-sbIL6P was a gift from Josep Planas (Addgene plasmid # 65099 ; http://n2t.net/addgene:65099 ; RRID:Addgene_65099)
  • For your References section:

    Transcriptional regulation of the gilthead seabream (Sparus aurata) interleukin-6 gene promoter. Castellana B, Marin-Juez R, Planas JV. Fish Shellfish Immunol. 2013 Jul;35(1):71-8. doi: 10.1016/j.fsi.2013.04.012. Epub 2013 Apr 18. 10.1016/j.fsi.2013.04.012 PubMed 23602849