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(Plasmid #65471)


Item Catalog # Description Quantity Price (USD)
Plasmid 65471 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Life Technologies
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Insert Size (bp)
  • Entrez Gene
    clpS (a.k.a. b0881, ECK0872, JW0865, yljA)
  • Promoter trc

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGTGGGCACTCGACCGGAATTATC
  • 3′ sequencing primer GGCGACACGGAAATGTTGAATAC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKS043 was a gift from Keith Tyo (Addgene plasmid # 65471 ; ; RRID:Addgene_65471)
  • For your References section:

    N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. Sekar K, Gentile AM, Bostick JW, Tyo KE. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016. PONE-D-15-42990 [pii] PubMed 26900850