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Pol
(Plasmid #65520)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65520 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Total vector size (bp) 8295
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HBV polymerase protein
  • Alt name
    HBx
  • Species
    Hepatitis B virus
  • Insert Size (bp)
    3466
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pol was a gift from Wang-Shick Ryu (Addgene plasmid # 65520 ; http://n2t.net/addgene:65520 ; RRID:Addgene_65520)
  • For your References section:

    Hepatitis B virus polymerase suppresses translation of pregenomic RNA via a mechanism involving its interaction with 5' stem-loop structure. Ryu DK, Kim S, Ryu WS. Virology. 2008 Mar 30;373(1):112-23. Epub 2007 Dec 21. 10.1016/j.virol.2007.11.010 PubMed 18155120