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BPK2101
(Plasmid #65770)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65770 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYCDuet-1
  • Backbone manufacturer
    EMD Millipore
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag, and SaCas9 gRNA
  • Promoter T7 (x2)
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACYC-DuetUP1
  • 3′ sequencing primer oBK390 (5'-GCAAATTCGACCCGGTCGTCG-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Use BsaI sites to insert sgRNA spacer sequence into the vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPK2101 was a gift from Keith Joung (Addgene plasmid # 65770 ; http://n2t.net/addgene:65770 ; RRID:Addgene_65770)
  • For your References section:

    Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK. Nature. 2015 Jun 22. doi: 10.1038/nature14592. 10.1038/nature14592 PubMed 26098369