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XW83
(Plasmid #65841)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65841 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CM290 (a derivative of pCFJ178)
  • Backbone manufacturer
    C. Maeder
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OR74A quinic acid utilization activator (qa-1F)
  • Alt name
    QF
  • Species
    Neurospora crassa
  • Insert Size (bp)
    2451
  • Mutation
    The endogenous SphI, KpnI and SacI sites in QF are removed or the convenience of cloning, most sites are compatible with pPD95.77
  • GenBank ID
    XM_954525.2
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Pmig-13::QF::SL2::mCherry fragment from XW43 was subcloned into SphI and PacI sites of CM290 (a derivative of pCFJ178, with extra cloning sites, a kind gift from C. Maeder), which is the chromosome IV cxTi10882 site MosSCI targeting vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XW83 was a gift from Kang Shen (Addgene plasmid # 65841 ; http://n2t.net/addgene:65841 ; RRID:Addgene_65841)
  • For your References section:

    Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans. Wei X, Potter CJ, Luo L, Shen K. Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929. 10.1038/nmeth.1929 PubMed 22406855