PurposeLuciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65880||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerPromega, modified by the Tuschl lab
- Backbone size w/o insert (bp) 6249
Modifications to backboneDEST-LR: includes LR clonase sites to introduce insert
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameCA repeats 200-300 nucleotides in length
Insert Size (bp)250
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 EEV (Common Sequencing Primers)
Terms and Licenses
LR recombinase reaction from Addgene plasmid 65869
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psicheck_200_300_CA_repeat was a gift from Thomas Tuschl (Addgene plasmid # 65880 ; http://n2t.net/addgene:65880 ; RRID:Addgene_65880)