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pETDuet-1∆HisTag_hNMT_Nef
(Plasmid #66078)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66078 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone size w/o insert (bp) 5420
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    NMT
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of first 80 amino acids
  • Entrez Gene
    NMT1 (a.k.a. HsNMT1, NMT)
  • Promoter T7-lacO

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer pBRrevBam
  • 3′ sequencing primer T7 Terminal
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Nef
  • Alt name
    Stamm SF2
  • GenBank ID
    AAB59883.1
  • Promoter T7-lacO

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site MunI (unknown if destroyed)
  • 5′ sequencing primer AmpR
  • 3′ sequencing primer pBR322ori-3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-1∆HisTag_hNMT_Nef was a gift from Dieter Willbold (Addgene plasmid # 66078 ; http://n2t.net/addgene:66078 ; RRID:Addgene_66078)
  • For your References section:

    Single vector system for efficient N-myristoylation of recombinant proteins in E. coli. Gluck JM, Hoffmann S, Koenig BW, Willbold D. PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081. 10.1371/journal.pone.0010081 PubMed 20404920