Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCAG aconitase GFP
(Plasmid #66104)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66104 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8147
  • Modifications to backbone
    The vector pCIG (pCAGGS-IRES2-nucEGFP) containing CMV enhancer/chicken b-actin promoter and rabbit b-globin polyA was double digested using ClaI and NotI to eliminate the IRES-nucGFP sequence.
  • Vector type
    Chicken expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    aconitase
  • Species
    chicken
  • Insert Size (bp)
    3147
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Aconitase insert contains W7L, L8A and A121V compared to reference sequence NM_204188.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG aconitase GFP was a gift from Felicitas Proels & Martin Scaal (Addgene plasmid # 66104 ; http://n2t.net/addgene:66104 ; RRID:Addgene_66104)
  • For your References section:

    Communication between distant epithelial cells by filopodia-like protrusions during embryonic development. Sagar, Prols F, Wiegreffe C, Scaal M. Development. 2015 Feb 15;142(4):665-71. doi: 10.1242/dev.115964. Epub 2015 Jan 23. 10.1242/dev.115964 PubMed 25617437