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YFP(1-158)-STOP
(Plasmid #66107)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66107 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNAI/mp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 5242
  • Modifications to backbone
    A BglII site was added to the polylinker 3' to the BamHI site.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP(1-158)STOP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    483
  • Mutation
    Gln69 was changed to Met.
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

YFP(1-158)Stop was amplified by PCR from EYFP (Clontech).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP(1-158)-STOP was a gift from Catherine Berlot (Addgene plasmid # 66107 ; http://n2t.net/addgene:66107 ; RRID:Addgene_66107)
  • For your References section:

    Regulation of expression and function of scavenger receptor class B, type I (SR-BI) by Na+/H+ exchanger regulatory factors (NHERFs). Hu Z, Hu J, Zhang Z, Shen WJ, Yun CC, Berlot CH, Kraemer FB, Azhar S. J Biol Chem. 2013 Apr 19;288(16):11416-35. doi: 10.1074/jbc.M112.437368. Epub 2013 Mar 12. 10.1074/jbc.M112.437368 PubMed 23482569