pECFP-Sec23B
(Plasmid
#66615)
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PurposeExpresses Sec16B as a CFP fusion
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepECFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec23B
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SpeciesM. musculus (mouse)
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MutationA2S, T3S, Y4S, L5F
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GenBank IDAAH05464.1
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Tag
/ Fusion Protein
- CFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor states that mutations do not not affect function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pECFP-Sec23B was a gift from David Stephens (Addgene plasmid # 66615 ; http://n2t.net/addgene:66615 ; RRID:Addgene_66615) -
For your References section:
Characterization of human Sec16B: indications of specialized, non-redundant functions. Budnik A, Heesom KJ, Stephens DJ. Sci Rep. 2011;1:77. doi: 10.1038/srep00077. Epub 2011 Aug 30. 10.1038/srep00077 PubMed 22355596