pBGSA FPR1 Q258A
(Plasmid #66724)

• Purpose: Constitutive expression of FPR1 Q258A in mammalian cells.
• Depositing Lab: Heini Miettinen
• Publication: Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Backbone

• Vector backbone: pBSSK+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 7200
• Total vector size (bp): 8520
• Modifications to backbone: Extensively modified
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FPR1 (haplotype 3)
• Alt name: FMLP-R26
• Species: H. sapiens (human)
• Insert Size (bp): 1320
• Mutation: Q258A
• GenBank ID: M60627
• Entrez Gene: FPR1 (a.k.a. FMLP, FPR)
• Promoter: SR-alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: 5'-CTTCTAGGCCTGTACGG-3'
• 3′ sequencing primer: 5'-TCTAGAACTAGTGGATCC-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This mutant shows constitutive chemotaxis and beta-arrestin binding. Also associated with PMID: 16038804.