pBZSA FPR1 ECFP
(Plasmid #66725)

• Purpose: Constitutive expression in mammalian cells
• Depositing Lab: Heini Miettinen
• Publication: Unpublished pBGSA FPR1 fluorophore tagged CDNAs (unpublished)

Backbone

• Vector backbone: pBSSK+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7560
• Modifications to backbone: Extensively modified
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Also resistant to Zeocin/Bleocin
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FPR1 (haplotype 3)-ECFP
• Species: H. sapiens (human)
• Insert Size (bp): 2060
• GenBank ID: M60627
• Entrez Gene: FPR1 (a.k.a. FMLP, FPR)
• Promoter: SR-alpha
• Tag / Fusion Protein:
  • ECFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: 5'-CTTCTAGGCCTGTACGG-3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ECFP was amplified from pECFP-C1 Clontech catalog #6076-1.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To create pBZSA, the neomycin-resistance gene was replaced with the zeocin-resistance gene (amplified as a SalI-SalI product from pZeo SV, Life Technologies.