PurposeEncodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66950||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBS SK
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 6479
Vector typeMammalian Expression, Mouse Targeting, CRISPR, TALEN
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1300
- Promoter beta-actin
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBSR (mCherry-surrogateTarget-bsr) was a gift from Silvestro Conticello (Addgene plasmid # 66950 ; http://n2t.net/addgene:66950 ; RRID:Addgene_66950)
For your References section:An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Niccheri F, Pecori R, Conticello SG. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. 10.1007/s00018-017-2524-y [pii] PubMed 28421278