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pBSR (mCherry-surrogateTarget-bsr)
(Plasmid #66950)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66950 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS SK
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 6479
  • Vector type
    Mammalian Expression, Mouse Targeting, CRISPR, TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    blasticidin
  • Insert Size (bp)
    1300
  • GenBank ID
  • Promoter beta-actin

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSR (mCherry-surrogateTarget-bsr) was a gift from Silvestro Conticello (Addgene plasmid # 66950 ; http://n2t.net/addgene:66950 ; RRID:Addgene_66950)
  • For your References section:

    An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Niccheri F, Pecori R, Conticello SG. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. 10.1007/s00018-017-2524-y [pii] PubMed 28421278