HaloTag-A2A-adenosine receptor
(Plasmid #66995)

• Purpose: A2A-adenosine receptor tagged with HaloTag on amino terminus
• Depositing Lab: Catherine Berlot
• Publication: Berlot Lab HaloTag Fusions (unpublished)

Backbone

• Vector backbone: Derived from pHT2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3988
• Total vector size (bp): 6286
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HaloTag-A2A-adenosine receptor
• Alt name: ADORA2A
• Species: H. sapiens (human)
• Insert Size (bp): 2298
• Mutation: The signal sequence from human growth hormone was added to the N-terminus of HaloTag, which was then added to the amino terminus of the A2A-adenosine receptor.
• Entrez Gene: ADORA2A (a.k.a. A2aR, ADORA2, RDC8)
• Promoter: CMV
• Tag / Fusion Protein:
  • Human Growth hormone Signal Sequence- HaloTag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EBV Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A2A-adenosine receptor cDNA was purchased from Guthrie cDNA Resource Center, Sayre PA (now Missouri S&T cDNA Resource Center, Rolla, MO). HaloTag pHT2 was purchased from Promega.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A2A-adenosine receptor insert (without the signal sequence) can be retrieved using Ngo MIV.