pSUP202pol4
(Plasmid
#67184)
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Purpose(Empty Backbone) Conjugative cloning vector
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSUP202pol2
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Backbone manufacturerGöttfert et al., Mol. Plant-Microbe Interact. 5:257-265, 1992
- Backbone size (bp) 7925
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Modifications to backboneEcoRI digestion of pSUP202pol2 followed by recircularization of a 6827 bp fragment
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Vector typeconjugative bacterial cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pSUP202pol2, which served as starting material for construction of pSUP202pol4, is derived from pSUP202 (see Göttfert et al., Mol. Plant-Microbe Interact. 5:257-265, 1992).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUP202pol4 was a gift from Hauke Hennecke & Julia Vorholt (Addgene plasmid # 67184 ; http://n2t.net/addgene:67184 ; RRID:Addgene_67184) -
For your References section:
One member of a gro-ESL-like chaperonin multigene family in Bradyrhizobium japonicum is co-regulated with symbiotic nitrogen fixation genes. Fischer HM, Babst M, Kaspar T, Acuna G, Arigoni F, Hennecke H. EMBO J. 1993 Jul;12(7):2901-12. PubMed 8101485