pMJH65
(Plasmid #67273)

• Purpose: Conjugally transferable red recombinogenic plasmid
• Depositing Lab: Mark Liles
• Publication: Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-35. doi: 10.1016/j.btre.2015.08.005. eCollection 2015 Dec.

Backbone

• Vector backbone: pMJH46
• Backbone size w/o insert (bp): 8754
• Total vector size (bp): 10254
• Vector type: Bacterial Expression ; Red Recombinase, temperature sensitive

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): SM10lambdapir
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: tetA
• Alt name: Tetracycline resistance gene
• Insert Size (bp): 1500
• Promoter: tetA promoter of plasmid pACYC184 origin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstZ17I (unknown if destroyed)
• 3′ cloning site: SfiI (unknown if destroyed)
• 5′ sequencing primer: GTAGACTTCCGTTGAACT
• 3′ sequencing primer: TAATGAATCGGCCAAC

Resource Information

• Supplemental Documents:
  • GenBank: KF195927
  • KF195927.1.gb
  • KF195927.1.dna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Conjugally transferable red recombinase vector pMJH65