pfbiRS
(Plasmid #67283)

• Purpose: Expresses the fbiC gene from Mycobacterium smegmatis
• Depositing Lab: Aziz Sancar
• Publication: Selby et al Biochemistry. 2012 Jan 10;51(1):167-71. doi: 10.1021/bi201536w. Epub 2011 Dec 27.

Backbone

• Vector backbone: pRSFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3829
• Total vector size (bp): 6500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use strain BL21 for induction/expression
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: fbiC gene from Mycobacterium smegmatis
• Alt name: Fo synthase
• Species: Mycobacterium smegmatis
• Insert Size (bp): 2700
• Mutation: non known
• GenBank ID: NC_008596.1
• Tag / Fusion Protein:
  • polyhistidine (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: LacI-R
• 3′ sequencing primer: DuetUP2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene is induced with IPTG. Following induction, Fo present in media.