PurposeTo quantify miR-141 function
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67632||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name2 binding sites for miR-141
SpeciesM. musculus (mouse)
Insert Size (bp)63
- Promoter SV40
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GCTGAAGAACGAGCAGTAAT
- 3′ sequencing primer GTCAGACAAACCCTAACCAC (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:miR-141 reporter was a gift from Judy Lieberman (Addgene plasmid # 67632 ; http://n2t.net/addgene:67632 ; RRID:Addgene_67632)
For your References section:miR-200-containing extracellular vesicles promote breast cancer cell metastasis. Le MT, Hamar P, Guo C, Basar E, Perdigao-Henriques R, Balaj L, Lieberman J. J Clin Invest. 2014 Dec;124(12):5109-28. doi: 10.1172/JCI75695. Epub 2014 Nov 17. 10.1172/JCI75695 PubMed 25401471