PurposeMiddle entry nuclear localized tdTomato reporter with 6xMT and 2A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67685||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Proteins
- 6xMyc-NLS (N terminal on insert)
- 2A (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME-6MTnlstdTomato2A was a gift from Nathan Lawson (Addgene plasmid # 67685)
For your References section:Vegfa signals through ERK to promote angiogenesis, but not artery differentiation. Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND. Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30. 10.1242/dev.137919 PubMed 27578780