pSG5-hPXR-K62Q
(Plasmid
#67766)
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PurposeExpression hPXR mutant K62Q in Mammalian Cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSG5
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 5405
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman pregnane X receptor (PXR)
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SpeciesH. sapiens (human)
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Insert Size (bp)1305
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Mutationlysine 62 to Glutamine
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GenBank IDNM_003889.3 NM_003889.3
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Entrez GeneNR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (unknown if destroyed)
- 3′ cloning site BamH1 (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-hPXR-K62Q was a gift from Sridhar Mani (Addgene plasmid # 67766 ; http://n2t.net/addgene:67766 ; RRID:Addgene_67766)
