pGEX-6P1-hPXR-LBD
(Plasmid #67769)

• Purpose: Expression GST-hPXR-LBD in bacteria
• Depositing Lab: Sridhar Mani
• Publication: Li et al J Biol Chem. 2013 May 10;288(19):13655-68. doi: 10.1074/jbc.M113.455485. Epub 2013 Mar 22.

Backbone

• Vector backbone: pGEX-6P1
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 5968
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human pregnane X receptor (PXR) ligand binding domain
• Species: H. sapiens (human)
• Insert Size (bp): 984
• Mutation: Contains amino acids 107 to 434 of human PXR
• GenBank ID: NM_003889.3 NM_003889.3
• Entrez Gene: NR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
• Promoter: Tac
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: pGEX Forward Primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry