PurposeUse together with VSV-G to package M-PMV reporter virus
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67794||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepSARM (full length M-PMV cone without Env gene)
Growth in Bacteria
Gene/Insert namefirefly luciferase
Entrez Genegag (a.k.a. MPMVgp2)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site BlpI (unknown if destroyed)
- 5′ sequencing primer Unknown
- 3′ sequencing primer ffLuc-R (Common Sequencing Primers)
Mason Pfizer monkey virus (M-PPMV) env gene is replaced with firefly luciferase
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSARM-luciferase was a gift from Stephen Goff (Addgene plasmid # 67794 ; http://n2t.net/addgene:67794 ; RRID:Addgene_67794)
For your References section:Postentry restriction of Mason-Pfizer monkey virus in mouse cells. Wang GZ, Goff SP. J Virol. 2015 Mar;89(5):2813-9. doi: 10.1128/JVI.03051-14. Epub 2014 Dec 24. 10.1128/JVI.03051-14 PubMed 25540373