Purposeluciferase reporter with mouse Id1 promoter fragments
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67811||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
SpeciesM. musculus (mouse)
- Promoter Id1/SV40
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p4XBRE-SBE-SV40-GL3 was a gift from Ron Prywes (Addgene plasmid # 67811 ; http://n2t.net/addgene:67811 ; RRID:Addgene_67811)
For your References section:Serum regulation of Id1 expression by a BMP pathway and BMP responsive element. Lewis TC, Prywes R. Biochim Biophys Acta. 2013 Oct;1829(10):1147-59. doi: 10.1016/j.bbagrm.2013.08.002. Epub 2013 Aug 13. 10.1016/j.bbagrm.2013.08.002 PubMed 23948603