|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67834||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerNovagen (EMD Millipore)
- Backbone size w/o insert (bp) 4671
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsDH5alpha is used for growth and propagation, but protein expression requires strain BL21 DE3
SpeciesM. musculus (mouse)
MutationFull-length mEKLF is aa 20-376; amino acid 19 is the initiator Methionine; See depositor comments below on mutations A236T, G299S, T305S
Entrez GeneKlf1 (a.k.a. Eklf, Nan)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
The Nde1 site contains the initiator methionine of murine EKLF (entire EKLF open reading frame is defined as 71-1215 and described in PMID 7682653). Mutations A236T, G299S, and T305S have no functional consequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET14b-mEKLF was a gift from James Bieker (Addgene plasmid # 67834 ; http://n2t.net/addgene:67834 ; RRID:Addgene_67834)
For your References section:A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro. Armstrong JA, Bieker JJ, Emerson BM. Cell. 1998 Oct 2;95(1):93-104. S0092-8674(00)81785-7 [pii] PubMed 9778250