pET14b-mEKLF
(Plasmid #67834)

• Purpose: Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution
• Depositing Lab: James Bieker
• Publication: Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

Backbone

• Vector backbone: pET14b
• Backbone manufacturer: Novagen (EMD Millipore)
• Backbone size w/o insert (bp): 4671
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha is used for growth and propagation, but protein expression requires strain BL21 DE3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EKLF
• Species: M. musculus (mouse)
• Mutation: Full-length mEKLF is aa 20-376; amino acid 19 is the initiator Methionine; See depositor comments below on mutations A236T, G299S, T305S
• Entrez Gene: Klf1 (a.k.a. Eklf, Nan)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Nde1 site contains the initiator methionine of murine EKLF (entire EKLF open reading frame is defined as 71-1215 and described in PMID 7682653). Mutations A236T, G299S, and T305S have no functional consequences.