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pET14b-mEKLF
(Plasmid #67834)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67834 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Backbone size w/o insert (bp) 4671
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha is used for growth and propagation, but protein expression requires strain BL21 DE3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EKLF
  • Species
    M. musculus (mouse)
  • Mutation
    Full-length mEKLF is aa 20-376; amino acid 19 is the initiator Methionine; See depositor comments below on mutations A236T, G299S, T305S
  • Entrez Gene
    Klf1 (a.k.a. Eklf, Nan)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The Nde1 site contains the initiator methionine of murine EKLF (entire EKLF open reading frame is defined as 71-1215 and described in PMID 7682653). Mutations A236T, G299S, and T305S have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET14b-mEKLF was a gift from James Bieker (Addgene plasmid # 67834 ; http://n2t.net/addgene:67834 ; RRID:Addgene_67834)
  • For your References section:

    A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro. Armstrong JA, Bieker JJ, Emerson BM. Cell. 1998 Oct 2;95(1):93-104. S0092-8674(00)81785-7 [pii] PubMed 9778250