PurposeEncodes GST fusion to murine EKLF (nucleotides 71-1215)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67836||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4969
Vector typeBacterial Expression
Growth in Bacteria
SpeciesM. musculus (mouse)
MutationFull-length mEKLF is aa 20-376; amino acid 19 is the initiator Methionine; See depositor comments below on mutations G299S, T305S
Entrez GeneKlf1 (a.k.a. Eklf, Nan)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pGEX5
- 3′ sequencing primer AmpR (ATAATACCGCGCCACATAGC) (Common Sequencing Primers)
Terms and Licenses
Mutations G299S, and T305S have no functional consequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-2TK-GST-mEKLF was a gift from James Bieker (Addgene plasmid # 67836 ; http://n2t.net/addgene:67836 ; RRID:Addgene_67836)
For your References section:Acetylation and modulation of erythroid Kruppel-like factor (EKLF) activity by interaction with histone acetyltransferases. Zhang W, Bieker JJ. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9855-60. PubMed 9707565