pABD933
(Plasmid
#67917)
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PurposeEntry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneGateway® pDONR™221
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Backbone manufacturerInvitrogen; life technologies Thermo Fisher Scientific
- Backbone size w/o insert (bp) 4761
- Total vector size (bp) 5913
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Vector typeGateway cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCTG10
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Alt nameCOLD TEMPERATURE GERMINATING 10
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1152
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GenBank IDDQ666277
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward (-20)
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pABD933 was a gift from Bruce Downie (Addgene plasmid # 67917 ; http://n2t.net/addgene:67917 ; RRID:Addgene_67917) -
For your References section:
KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1. Majee M, Kumar S, Kathare PK, Wu S, Gingerich D, Nayak NR, Salaita L, Dinkins R, Martin K, Goodin M, Dirk LMA, Lloyd TD, Zhu L, Chappell J, Hunt AG, Vierstra R, Huq E, Downie AB. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 10.1073/pnas.1711919115. Epub 2018 Apr 9. 10.1073/pnas.1711919115 PubMed 29632208