mouse E-cadherin GFP (423)
(Plasmid #67937)

• Purpose: Mammalian expression of E-cadherin-GFP
• Depositing Lab: Alpha Yap
• Publication: Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-32. doi: 10.1242/jcs.134379. Epub 2014 Mar 20.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E-cadherin
• Alt name: cadherin 1
• Alt name: Cdh1
• Species: M. musculus (mouse)
• Insert Size (bp): 3000
• Entrez Gene: Cdh1 (a.k.a. ARC-1, E-cad, Ecad, L-CAM, UVO, Um)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry