pcDNA4_ChR2opt_mKATE
(Plasmid #67958)

• Purpose: optical depolarization of mammalian cells
• Depositing Lab: Andreas Offenhäusser
• Publication: ChR2opt_mKATE (unpublished)

Backbone

• Vector backbone: pcDNA4/TO
• Backbone manufacturer: Invitrogen (TRex Kit)
• Backbone size w/o insert (bp): 5078
• Total vector size (bp): 6833
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Channelrhodopsin 2 opt
• Alt name: ChR2opt
• Species: C. reinhardtii
• Insert Size (bp): 1761
• Mutation: synthetically produced for mammalian optimized coding; H134R *(see below)
• Promoter: CMV -TetON
• Tag / Fusion Protein:
  • mKatushka (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMVfor; CGAATTTAAAAACCCCAGCA; GTCCAAGGGCGAGGAACT
• 3′ sequencing primer: TGCTGTAGTCGTTGCTCAGG; CCTTCCACCACCTTGATCT; GATGGCTGGCAACTAGAAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was synthetically produced by GeneArt and supplied in pcDNA4/TO from Invitrogen
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note: This plasmid contains a standard, published H134R variant of Channelrhodopsin 2 that is reported to increase the currents achieved by the channel (see Nagel et al. 2005).