pABD1275
(Plasmid #68017)

• Purpose: PIF1 protein overproduction for purification from bacterial cells
• Depositing Lab: Bruce Downie
• Publication: Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 10.1073/pnas.1711919115. Epub 2018 Apr 9.

Backbone

• Vector backbone: pET23b
• Backbone manufacturer: Novagen; EMD Millipore
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 5109
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PHY-INTERACTING FACTOR 1 (PIF1)
• Alt name: PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 (PIL5)
• Species: A. thaliana (mustard weed); AT2G20180
• Insert Size (bp): 1443
• Mutation: Codon Optimized for bacterial expression (GenScript)
• Entrez Gene: PIL5 (a.k.a. AT2G20180, PHY-INTERACTING FACTOR 1, PIF1, T2G17.2, T2G17_2, phytochrome interacting factor 3-like 5)
• Promoter: T7
• Tag / Fusion Protein:
  • 6x-His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry