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Purpose(Empty Backbone) Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMpGWB300
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Backbone manufacturerLaboratory of Plant Molecular Biology, Graduate School of Biostudies, Kyoto University
- Backbone size (bp) 10455
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Vector typePlant Expression, Cre/Lox
- Promoter MpEF1alpha promoter
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Selectable markersChlorsulfuron
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMpGWB337 was a gift from Takayuki Kohchi (Addgene plasmid # 68061 ; http://n2t.net/addgene:68061 ; RRID:Addgene_68061) -
For your References section:
Conditional Gene Expression/Deletion Systems for Marchantia polymorpha Using its Own Heat-shock Promoter and the Cre/loxP-mediated Site-specific Recombination. Nishihama R, Ishida S, Urawa H, Kamei Y, Kohchi T. Plant Cell Physiol. 2015 Jul 6. pii: pcv102. 10.1093/pcp/pcv102 PubMed 26148498
Map uploaded by the depositor.
