pCAG-GW-V5-Hygro
(Plasmid
#68265)
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Purpose(Empty Backbone) Constitutive selectable expression of C-term V5 tagged proteins
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAGS644 pCAGS-IRES-Hygro
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Backbone manufacturerAustin Smith, University of Cambridge
- Backbone size (bp) 8563
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Vector typeMammalian Expression
- Promoter CAG
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Addgene's quality control sequencing identified a Y64H amino acid residue substitution in the Hygromycin resistance cassette. This residue change is not thought to affect the plasmids function or ability to confer resistance to Hygromycin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-GW-V5-Hygro was a gift from Mitchell Guttman (Addgene plasmid # 68265 ; http://n2t.net/addgene:68265 ; RRID:Addgene_68265) -
For your References section:
The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3. McHugh CA, Chen CK, Chow A, Surka CF, Tran C, McDonel P, Pandya-Jones A, Blanco M, Burghard C, Moradian A, Sweredoski MJ, Shishkin AA, Su J, Lander ES, Hess S, Plath K, Guttman M. Nature. 2015 May 14;521(7551):232-6. doi: 10.1038/nature14443. Epub 2015 Apr 27. 10.1038/nature14443 PubMed 25915022