pKLV2-EF1a-Cas9Bsd-W Citations (20)
Originally described in: A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. PubMed Journal
Articles Citing pKLV2-EF1a-Cas9Bsd-W
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| Genome-scale identification of cellular pathways required for cell surface recognition. Sharma S, Bartholdson SJ, Couch ACM, Yusa K, Wright GJ. Genome Res. 2018 Sep;28(9):1372-1382. doi: 10.1101/gr.231183.117. Epub 2018 Jun 18. PubMed |
| Unsupervised correction of gene-independent cell responses to CRISPR-Cas9 targeting. Iorio F, Behan FM, Goncalves E, Bhosle SG, Chen E, Shepherd R, Beaver C, Ansari R, Pooley R, Wilkinson P, Harper S, Butler AP, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ. BMC Genomics. 2018 Aug 13;19(1):604. doi: 10.1186/s12864-018-4989-y. PubMed |
| Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens. Behan FM, Iorio F, Picco G, Goncalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ. Nature. 2019 Apr;568(7753):511-516. doi: 10.1038/s41586-019-1103-9. Epub 2019 Apr 10. PubMed |
| A molecular triage process mediated by RING finger protein 126 and BCL2-associated athanogene 6 regulates degradation of G0/G1 switch gene 2. Kamikubo K, Kato H, Kioka H, Yamazaki S, Tsukamoto O, Nishida Y, Asano Y, Imamura H, Kawahara H, Shintani Y, Takashima S. J Biol Chem. 2019 Oct 4;294(40):14562-14573. doi: 10.1074/jbc.RA119.008544. Epub 2019 Aug 1. PubMed |
| Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity. Liao JQ, Zhou G, Zhou Y. Methods Mol Biol. 2020 Jun 22. doi: 10.1007/7651_2020_304. PubMed |
| Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens. Sharma S, Wright GJ. J Vis Exp. 2020 Jun 6;(160). doi: 10.3791/60803. PubMed |
| Analysis of CRISPR-Cas9 screens identifies genetic dependencies in melanoma. Christodoulou E, Rashid M, Pacini C, Droop A, Robertson H, Groningen TV, Teunisse AFAS, Iorio F, Jochemsen AG, Adams DJ, Doorn RV. Pigment Cell Melanoma Res. 2021 Jan;34(1):122-131. doi: 10.1111/pcmr.12919. Epub 2020 Sep 7. PubMed |
| CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation. Hsu J, Huang HT, Lee CT, Choudhuri A, Wilson NK, Abraham BJ, Moignard V, Kucinski I, Yu S, Hyde RK, Tober J, Cai X, Li Y, Guo Y, Yang S, Superdock M, Trompouki E, Calero-Nieto FJ, Ghamari A, Jiang J, Gao P, Gao L, Nguyen V, Robertson AL, Durand EM, Kathrein KL, Aifantis I, Gerber SA, Tong W, Tan K, Cantor AB, Zhou Y, Liu PP, Young RA, Gottgens B, Speck NA, Zon LI. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23626-23635. doi: 10.1073/pnas.2003228117. Epub 2020 Sep 3. PubMed |
Genome-wide CRISPR screens of oral squamous cell carcinoma reveal fitness genes in the Hippo pathway.
Chai AWY, Yee PS, Price S, Yee SM, Lee HM, Tiong VK, Goncalves E, Behan FM, Bateson J, Gilbert J, Tan AC, McDermott U, Garnett MJ, Cheong SC.
Elife. 2020 Sep 29;9. pii: 57761. doi: 10.7554/eLife.57761.
PubMed
Associated Plasmids |
| Combinatorial CRISPR screen identifies fitness effects of gene paralogues. Thompson NA, Ranzani M, van der Weyden L, Iyer V, Offord V, Droop A, Behan F, Goncalves E, Speak A, Iorio F, Hewinson J, Harle V, Robertson H, Anderson E, Fu B, Yang F, Zagnoli-Vieira G, Chapman P, Del Castillo Velasco-Herrera M, Garnett MJ, Jackson SP, Adams DJ. Nat Commun. 2021 Feb 26;12(1):1302. doi: 10.1038/s41467-021-21478-9. PubMed |
| Werner Helicase Is a Synthetic-Lethal Vulnerability in Mismatch Repair-Deficient Colorectal Cancer Refractory to Targeted Therapies, Chemotherapy, and Immunotherapy. Picco G, Cattaneo CM, van Vliet EJ, Crisafulli G, Rospo G, Consonni S, Vieira SF, Rodriguez IS, Cancelliere C, Banerjee R, Schipper LJ, Oddo D, Dijkstra KK, Cinatl J, Michaelis M, Yang F, Di Nicolantonio F, Sartore-Bianchi A, Siena S, Arena S, Voest EE, Bardelli A, Garnett MJ. Cancer Discov. 2021 Aug;11(8):1923-1937. doi: 10.1158/2159-8290.CD-20-1508. Epub 2021 Apr 9. PubMed |
| Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia. Supper E, Rudat S, Iyer V, Droop A, Wong K, Spinella JF, Thomas P, Sauvageau G, Adams DJ, Wong CC. Nat Commun. 2021 Apr 30;12(1):2482. doi: 10.1038/s41467-021-22750-8. PubMed |
| Nuclear NAD(+) homeostasis governed by NMNAT1 prevents apoptosis of acute myeloid leukemia stem cells. Shi X, Jiang Y, Kitano A, Hu T, Murdaugh RL, Li Y, Hoegenauer KA, Chen R, Takahashi K, Nakada D. Sci Adv. 2021 Jul 21;7(30). pii: 7/30/eabf3895. doi: 10.1126/sciadv.abf3895. Print 2021 Jul. PubMed |
| Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2. Gu Y, Cao J, Zhang X, Gao H, Wang Y, Wang J, He J, Jiang X, Zhang J, Shen G, Yang J, Zheng X, Hu G, Zhu Y, Du S, Zhu Y, Zhang R, Xu J, Lan F, Qu D, Xu G, Zhao Y, Gao D, Xie Y, Luo M, Lu Z. Cell Res. 2022 Jan;32(1):24-37. doi: 10.1038/s41422-021-00595-6. Epub 2021 Nov 26. PubMed |
| Functional Genomic Identification of Predictors of Sensitivity and Mechanisms of Resistance to Multivalent Second-Generation TRAIL-R2 Agonists. Grinkevitch V, Wappett M, Crawford N, Price S, Lees A, McCann C, McAllister K, Prehn J, Young J, Bateson J, Gallagher L, Michaut M, Iyer V, Chatzipli A, Barthorpe S, Ciznadija D, Sloma I, Wesa A, Tice DA, Wessels L, Garnett M, Longley DB, McDermott U, McDade SS. Mol Cancer Ther. 2022 Apr 1;21(4):594-606. doi: 10.1158/1535-7163.MCT-21-0532. PubMed |
| Knowledge graph-based recommendation framework identifies drivers of resistance in EGFR mutant non-small cell lung cancer. Gogleva A, Polychronopoulos D, Pfeifer M, Poroshin V, Ughetto M, Martin MJ, Thorpe H, Bornot A, Smith PD, Sidders B, Dry JR, Ahdesmaki M, McDermott U, Papa E, Bulusu KC. Nat Commun. 2022 Mar 29;13(1):1667. doi: 10.1038/s41467-022-29292-7. PubMed |
| Novel Treatments of Uveal Melanoma Identified with a Synthetic Lethal CRISPR/Cas9 Screen. Glinkina K, Groenewoud A, Teunisse AFAS, Snaar-Jagalska BE, Jochemsen AG. Cancers (Basel). 2022 Jun 29;14(13):3186. doi: 10.3390/cancers14133186. PubMed |
| HIRA loss transforms FH-deficient cells. Valcarcel-Jimenez L, Rogerson C, Yong C, Schmidt C, Yang M, Cremades-Rodelgo M, Harle V, Offord V, Wong K, Mora A, Speed A, Caraffini V, Tran MGB, Maher ER, Stewart GD, Vanharanta S, Adams DJ, Frezza C. Sci Adv. 2022 Oct 21;8(42):eabq8297. doi: 10.1126/sciadv.abq8297. Epub 2022 Oct 21. PubMed |
| Expanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability. Chen ACH, Lee YL, Ruan H, Huang W, Fong SW, Tian S, Lee KC, Wu GM, Tan Y, Wong TCH, Wu J, Zhang W, Cao D, Chow JFC, Liu P, Yeung WSB. Adv Sci (Weinh). 2023 Apr;10(11):e2204797. doi: 10.1002/advs.202204797. Epub 2023 Feb 12. PubMed |
| BRCA2 Germline Mutations Identify Gastric Cancers Responsive to PARP Inhibitors. Petrelli A, Rizzolio S, Pietrantonio F, Bellomo SE, Benelli M, De Cecco L, Romagnoli D, Berrino E, Orru C, Ribisi S, Moya-Rull D, Migliore C, Conticelli D, Maina IM, Puliga E, Serra V, Pellegrino B, Llop-Guevara A, Musolino A, Siena S, Sartore-Bianchi A, Prisciandaro M, Morano F, Antista M, Fumagalli U, De Manzoni G, Degiuli M, Baiocchi GL, Amisano MF, Ferrero A, Marchio C, Corso S, Giordano S. Cancer Res. 2023 May 15;83(10):1699-1710. doi: 10.1158/0008-5472.CAN-22-2620. PubMed |
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