TriEx-4 DHX36
(Plasmid #68368)

• Purpose: Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.
• Depositing Lab: James Vaughn
• Publication: Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-20. Epub 2005 Sep 7.

Backbone

• Vector backbone: pTriEx-4
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 8264
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Growth instructions: For protein expression, use Rosetta 2 cells and selection plates as well as terrific broth should contain 50 µg/ml ampicillin (or carbenicillin) and 35 µg/ml chloramphenicol.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DHX36
• Alt name: G4R1
• Alt name: RHAU
• Species: H. sapiens (human)
• Insert Size (bp): 3026
• GenBank ID: NM_020865.2
• Entrez Gene: DHX36 (a.k.a. DDX36, G4R1, MLEL1, RHAU)
• Promoter: CMV ie enhancer/promoter
• Tag / Fusion Protein:
  • Histidine (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PshAI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: GTT ATT GTG CTG TCT CAT CA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For protein expression, use Rosetta 2 cells and selection plates as well as terrific broth should contain 50 µg/ml ampicillin (or carbenicillin) and 35 µg/ml chloramphenicol.