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PGR-Blue
(Plasmid #68374)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68374 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSB1A10
  • Backbone manufacturer
    http://partsregistry.org/partsdb/get_part.cgi?part=pSB1A10
  • Backbone size (bp) 5191
  • Modifications to backbone
    Added BsaI cut site and sticky ends for directional golden gate assembly. Left facing AmilCP (Blue Chromo Protein) added between GFP/RFP coding sequences.
  • Vector type
    Bacterial Expression, Synthetic Biology ; Golden Gate Assembly
  • Promoter pBAD

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Modification of PGR (Voigt Lab): http://www.addgene.org/46002/
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Instructions on how to use this plasmid can be found in the associated JoVE article: http://www.jove.com/video/54064.

Terminator quantification vector utilizing Golden Gate Assembly and amilCP for easy colony selection. Colonies containing ligated inserts are Green and colonies containing the uncut plasmid are blue. Synthesized terminators must contain left sticky end of 5’ CGAC 3’ and right sticky end of 5’ CCGC 3’. Terminators are placed between GFP and RFP and expression is driven by pBAD arabinose inducible promoter. This is a modification of the PGR plasmid developed by the Voigt Lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGR-Blue was a gift from Nathan Reyna (Addgene plasmid # 68374 ; http://n2t.net/addgene:68374 ; RRID:Addgene_68374)