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pU6_(GLuc)_INT[P4-P6(3xPP7)]
(Plasmid #68432)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68432 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNEB193
  • Backbone manufacturer
    NEB
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
  • gRNA/shRNA sequence
    gRNA: GATCTAGATACGACTCACTAT
  • Species
    Synthetic
  • Promoter human U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer LKO.1 5
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

sgRNA core targets the (ATCTAGATACGACTCACTAT) sequence in the Gluc reporter. Contains an internal "accessory domain," comprising the T. thermophilia P4-P6 domain appended with a casette of PP7 stem-loops.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6_(GLuc)_INT[P4-P6(3xPP7)] was a gift from John Rinn (Addgene plasmid # 68432 ; http://n2t.net/addgene:68432 ; RRID:Addgene_68432)
  • For your References section:

    Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. 10.1038/nmeth.3433 PubMed 26030444