Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed November 28th & 29th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of November 25th - 29th. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

TOPO ZeoR
(Plasmid #68446)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68446 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    TOPO 2-5
  • Backbone size w/o insert (bp) 3524
  • Vector type
    Bacterial Expression ; GMAP
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Zeocin Resistance
  • Alt name
    ZeoR
  • Insert Size (bp)
    378
  • Promoter Plac

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTAACTCGAACGCTAGCTGTGCGATCGTTT
  • 3′ sequencing primer AGAGTAATTCAACCCCAAACAACAACGTTT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TOPO ZeoR was a gift from Tyler Jacks (Addgene plasmid # 68446 ; http://n2t.net/addgene:68446 ; RRID:Addgene_68446)
  • For your References section:

    A Modular Assembly Platform for Rapid Generation of DNA Constructs. Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T. Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. 10.1038/srep16836 PubMed 26887506